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The segments were normalized to 90%% of the internal circumference a vessel would have at 50 mmHg.
The segment was normalized, attaining 90% of the internal circumference that a fully relaxed vessel would have under a transmural pressure of 100 mmHg or 50 mmHg (see below).
The vessels were set to the internal circumference L1, given by L1 = 0.9×L100.
The relationship between resting wall tension and internal circumference was determined, and the internal circumference, L100, corresponding to a transmural pressure of 100mmHg for a relaxed vessel in situ, was calculated.
An electromagnetic flow probe (Model 100 series, internal circumference = 8 mm; Carolina Medical Electronics, King, NC) was positioned around the ascending aorta to measure the pulsatile aortic flow.
Contraction data were calculated as active effective pressure using the following equation: Active effective pressure = (wall tension/2π)/vessel internal circumference.
Similar(50)
The internal vessel circumference of arteries taken from both ACTH-treated rats and SHR showed a trend towards a decrease, compared with WKY, although only saphenous arteries from SHR exhibited hypertrophy of the smooth muscle cell layers.
"BMI measures total fat; waist circumference measures internal fat," said study researcher Margaret Ashwell, with the Oxford Brookes University in England.
MNA is composed of eighteen items, such as content of meals, BMI, internal use and midarm circumference (MAC).
Vessel circumference at the internal elastic lamina, the number of smooth muscle cell layers and endothelial cell areas were determined for each vessel as previously described [ 48, 49].
For morphometric analyses, Metamorph Imaging 7.0 software (Molecular Devices) was used to measure external elastic lamina, internal elastic lamina, and lumen circumference as well as medial and neointimal area 21 days after injury.
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