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Lengthening the lentiviral (LV) vector transgene cassette by an additional 1.2 kb by an internal cassette caused no further reduction in titers.
Our final mouse dataset consisted of 29 416 transcripts and 4 964 internal cassette exons, and the human dataset of 79 030 transcripts and 11 664 internal cassette exons.
We used a Bayesian approach to estimate the fraction f of internal cassette exons whose inclusion depends on the choice of TSS.
For each transcription unit, we first identified (1) the set of internal cassette exons and (2) the set of transcription start sites (TSSs).
Both studies mainly considered internal cassette exons and reached similar conclusions, namely, that young exons tend to be alternatively spliced and minor.
In addition, an internal cassette allowed expression of the green fluorescent protein (GFP) marker gene under the control of the elongation factor (EF-1) α promoter [ 43].
Similar(46)
In the SIN context, gammaretroviral and lentiviral vectors with comparable internal cassettes had similar expression properties.
The methodology used for the bombardment assay in switchgrass and rice is the first plant transformation study of promoter expression using an internal LUC cassette control within the same vector backbone as the experimental promoter- GUS fusion cassette, as opposed to the standard use of co-transformation [ 19, 63, 64].
This finding indicates that an as-yet-undocumented event occurred among H9 strains to generate the internal gene cassette of the novel H7N9 lineage.
The H7N9 virus possesses internal gene cassettes from poultry H9N2 virus, another low pathogenicity virus that circulates widely in poultry in Asia (3, 5, 6 ).
On November, 2013, another newly reassorted avian H10N8 virus, which possesses internal gene cassettes recruited from the poultry H9N2 virus, was isolated from a 73 years old woman [ 16].
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