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Furthermore, the internal calibration function facility for each tool of the digital logging device was utilized prior to running the logs.
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Prior to imaging, a plate map and internal calibration were performed using Calmix1 Applied Biosystemss).
After the spectra data were recorded and transferred back to the tablet, the ratio of the selected fragment ions generated from the ionized drug and internal standard was calculated and used for determination of the drug concentration based on the corresponding calibration function, which was constructed using the same drug and internal standard and the same type of matrix.
By the use of this internal calibration mix the each individual compound was mass corrected.
Myoglobin digested with trypsin was used to calibrate the mass instrument with internal calibration mode.
External calibration was performed with a peptide calibration standard (Bruker Daltonics Billerica, Mass .USA, Part No.: 206195) and internal calibration with trypsin autoproteolytic fragments.
Protein digested by trypsin was used to calibrate the mass instrument using the internal calibration mode.
Quantification of derived signals was based on an external calibration function determined with aqueous standard solutions using Indium as internal standard.
Calibration was performed using internal calibration process.
Calibration was performed by using internal calibration process.
Three trypsin autolysis peaks were used for internal calibration.
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