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The calcium ionophore ionomycin (1 μM) was added at the end of each time course as an internal assay control.
As an internal assay control TNF-α mRNA expression was also determined.
Doubly cross-linked DnaN ('XX-DnaN') serves as internal assay control as visualized by immunoblotting.
The staining in normal elements and stroma was also used as a positive internal assay control, as applicable.
The radiation-induced G2 aberration frequencies for the transport and internal assay controls are shown in Table 1.
'XX-DnaN' serves as internal assay control visualized by immunoblotting of cross-linked species of DnaN protein (bottom panel).
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Quantification of the protected species from 30 μg was performed using a phosphoimager (Molecular Dynamics), and related to an internal control assay for the constitutively expressed U6 small nuclear RNA (LC), performed for each assay as described (Maxwell et al, 1999).
To obtain semiquantitative levels of telomerase activity, an internal telomerase assay standard (ITAS; 25 attograms), amplified by the same 2 primers used for the telomerase activity assay, was included in the TRAP buffer.
Both sets were multiplexed with a reliable internal control assay targeting host plant DNA, and their diagnostic specificity and sensitivity remained unchanged.
An internal control assay designed to target the rice ubiquitin 5 gene (UBQ5) was included in detecting RSV in different infected rice tissues.
Let-7b was used as housekeeping internal control (assay ID 000378).
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