The resulting tertiary structure was investigated using intermolecular and intramolecular variants of the same dimer.
Intermolecular and intramolecular variants show that the near-infrared cluster coordinates two DNA strands with antiparallel orientations.
The gel-forming mucins form intermolecular disulphide-linked multimers.
The authors concluded that the oligomerization domain, if indeed folded like a coiled-coil domain, was more likely to be involved in homomeric intermolecular interactions and that the S704C variant modulated this.
Three scFab variants with different linker length from 32 to 36 amino acids and one 34 amino acid variant with a deleted intermolecular disulphide bond (scFabΔC) were compared to scFv and Fab using the lysozyme binding antibody D1.3 [ 23, 24] as a model antibody.
In two-Cys peroxiredoxins, the relatively short-lived sulfenic cysteine reacts with a second, "resolving", cysteine to form a disulfide bridge, which is intermolecular in typical peroxiredoxins and intramolecular in atypical variants.
Conversely, stabilization of this crystal contact by two intermolecular disulfide bonds generates a constitutively dimeric InlB variant with exceptionally high signaling activity, which can stimulate cell motility and cell division.
Intriguingly, the three selected cysteine variants generated dimers by formation of intermolecular disulfide bridges.
However, this difference is not constant across the entire series of SeCobA variants investigated, because specific, species-dependent intermolecular interactions between the protein side chains and the DMB moiety are likely important for promoting Co N DMB) bond dissociation (vide supra).
The 2.2-Å X-ray structure of one insertion variant reveals that strain is relieved by intermolecular folding with an identically unfolded Bn domain, resulting in a domain-swapped polymer.
