To obtain additional information on the nature of the oligomers formed in the aggregation process, we explored the use of intermolecular fluorescence resonance energy transfer (FRET) measurements.
To date, much of what is known about oligomerization of β2ARs and other Family A GPCRs comes from elegant studies using intermolecular fluorescence/bioluminescence resonance energy transfer (FRET or BRET) in live cells (Angers et al, 2000; Salahpour et al, 2004; Milligan and Bouvier, 2005).
In addition to conventional fluorescent dyes, genetically engineered fluorescent proteins are increasingly being used to measure molecular conformations or intermolecular distances by fluorescence resonance energy transfer.
Analysis of the preassembled neuronal SNARE complexes by intermolecular single-molecule fluorescence resonance energy transfer (smFRET) revealed that they represent a mixture of parallel and antiparallel configurations involving the SNARE motifs of syntaxin and synaptobrevin and the SNARE motifs of syntaxin and SNAP-25.
The synthesis and crystal structure of 5- 2-chloro-ethyl -6-methyl-uracil 3 and 5- 2-chloro-ethyl -6-methyl-uraciluracil 4 are described, which revealed sulfur–π interaction including resonance-induced intermolecular H-bonding in 4 whereas compound 3 showed only strong H-bonding and formed a dimer.
Two resonances exhibit intermolecular NOEs (see below) to the DNA: one appears at δ=9.35 and the other at 9.14 ppm.
Mini-B and DEPN-8 had direct intermolecular interactions based on plasmon resonance binding affinity (Table 2) and on deconvolution analyses of FTIR spectra indicating a modified peptide secondary structure in multilayers with DEPN-8 (Fig. 1, Table 1).
Based on the double-quantum-filtered technique, a three-pulse sequence was designed to effectively eliminate contamination of residual conventional single-quantum coherences (SQC) from intermolecular SQC signals in single-resonance spin-1/2 samples such as water.
BIACORE, a surface plasmon resonance-based biosensor technology, allows intermolecular interactions to be measured in real time, and can provide both equilibrium and kinetic information about complex formation.
This paper presents an analytical study on the forced vibration of electrically actuated micro-switches near resonance region, taking into consideration the intermolecular force, axial residual stress, and geometrical nonlinearity due to mid-plane stretching.
