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No intermolecular complementation was observed.
We tested this latter possibility by investigating whether intermolecular complementation occurred between two mutant Rad60 proteins defective in one case in SLD1 and in the other in SLD2.
We investigated this model by intermolecular complementation assays using pairs of signaling-deficient LAT-1 variants.
Although the ability for intermolecular complementation sheds light on the 7TM-dependent activity of LAT-1, it does not account for the 7TM-independent function of the receptor.
This suggests that ligand-induced proximity of two LAT-1 receptors mediated by the RBL domain might be required for the intermolecular complementation of GPS and 7TM domains.
We excluded that DNA recombination between the transgenes accounted for the intermolecular complementation as we were unable to detect repaired constructs by PCR in rescued strains.
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This "complementation" depends to some extent on how genetically distinct the populations are.
(Complementation) b.
(D) Complementation plasmid.
Bimolecular fluorescence complementation.
Figure 4 Srs5 complementation test.
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