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The reaction intermediates were analyzed by HPLC- -ESI -TOF-MS spectroscopy whose result indicated the complete mineralization of PNP and PNA.
All cloning intermediates were analyzed by restriction digests and sequenced using the appropriate primers [see Additional file 2].
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Next, the protein composition of the affinity purified intermediates was analyzed by qMS as described above, using N-labeled 70S particles as a reference.
The residual dye and organic intermediates were analyzed using HPLC-PDA-ESI-MS and GC-MS.
The progress of degradation and intermediates formed were analyzed by HPLC and GC MS analysis.
The structures of intermediate polymers were analyzed by spectral methods (1H NMR, FTIR).
The electrochemical activity was investigated by polarization experiments and the reaction intermediates and products were analyzed by in situ Fourier Transform Infra-Red Spectroscopy (FTIR).
To this aim, cells were incubated with C6-glucose and the abundance of C-labelled TCA cycle intermediates and palmitate were analyzed by LCMS.
The modified oligonucleotides were then incubated with purified AlkB for 1 h at 37 °C, and the reaction products and intermediates (Scheme 1) were analyzed by high resolution ESI-TOF mass spectrometry.
The amount biodegraded and the intermediates formed during biodegradation were analyzed by HPLC and GC MS.
Plasma samples from 492 individuals (66 lowlands, 121 intermediate and 305 highlands) were analyzed by Enzyme Linked ImmunoSorbent Assays (ELISAs).
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