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The relative content of TCA cycle intermediates was determined by mass spectrometry.
To further define the metabolic state of gametocytes, Stage III gametocytes were cultured in the presence of C-glucose or C-glutamine, and enrichment in intracellular intermediates was determined by GC-MS.
Information about each substrate is provided in Supplementary file 2. DOI: http://dx.doi.org/10.7554/eLife.06492.009 To examine the genetic changes causing phenotypic reversion, the sequence of all evolutionary intermediates was determined.
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The formed intermediates were determined and the degradation mechanism was discussed.
Quantity of phenanthrene concentrations and varieties of catabolic intermediates were determined using HPLC.
The geometry and electronic structure of the implicated active sites and intermediates were determined.
Using mutants that isolated various tertiary interactions, the thermodynamic parameters of several ribozyme-substrate intermediates were determined.
The results of such studies reveal an underlying complexity, which is simply understood once one is cognizant of the 'chameleon'-like behavior of such intermediates is determined by the high-valent intermediate environment.
U.S.A. 95 9274 9279, 1998) for PEG-induced fusion of phosphatidylcholine liposomes supports the idea that subsequent to stable fusion pore formation, the evolution of fusion intermediates is determined more by the lipids than by the proteins.
Quantitative mole fractions of reactants, products and numerous stable and reactive intermediates were determined by electron ionization (EI) MBMS with high mass resolution to separate overlapping features from species with different heavy elements by exact mass.
The sizes of the intermediates were determined using a 2D Lorentzian function.
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