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The assignment of intermediates was confirmed by MS/MS fragmentation analysis.
The identity of the quinolone alkaloids and their corresponding intermediates was confirmed by analysis of 1D and 2D NMR spectroscopic, LC ESI MS and ESI-HRMS data.
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Delivery of intracellular succinate and anaplerosis of tricarboxylic acid (TCA) cycle intermediates were confirmed (Fig. 2a; Supplementary Fig. 3).
The structures of the AB2-type monomers and their intermediates were confirmed using FT-IR, 1H NMR, and 13C NMR spectroscopy.
Thioester-linked intermediates were confirmed by their susceptibility to reduction by DTT.
Examples of new sentences added for clarity are: "Thioester-linked intermediates were confirmed by their susceptibility to reduction by DTT.
Thioester-linked intermediates were confirmed by DTT-reduced controls collected by treating half of the last time-point samples with 100 mM DTT. E3∼Ub conjugates that accumulate for the defective mutants are thoroughly reduced with DTT except for the Lys432Ala/Arg433Ala/Asp434Ala/Arg436Ala and Arg437Ala/Lys438Ala/Ile440Ala/Tyr441Ala mutants (data not shown).
The formation of a cartilage intermediate was confirmed by histology.
The presence of this intermediate was confirmed by means of XPS measurements on potentiostatically etched surface.
The suggested formation of a high-energy intermediate was confirmed by incubation with 15-azasterols, one of which is a natural product, 15-aza-24-methylene 15-aza-24-methylene 15-aza-24-methylene 15-aza-24-methylene - d-homocholesta-8,14-dien-3β-ol d-homocholesta-8,14-dien-3β-ol d-homocholesta-8,14-dien-3β-ol d-homocholesta-8,14-dien-3β-ol d-homocholesta-8,14-dien-3β-ol d-homocholesta-8,14-dien-3β-ol
The existence of such an intermediate was confirmed by a reduced hydrophobicity of the tryptophan microenvironments which was evidenced by fluorescence spectra, as well as by the fact of a high content of native-like secondary structure as showing in CD spectra.
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