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Mv (median vectors representing missing intermediates) shows unsampled nodes inferred by MJ network analysis, and the number along the branches shows the position of mutations.

TruD thus works from the C-terminus within the core, but the accumulation of the intermediates shows that they are released and rebound, rather than held on the enzyme.

All these intermediates showed IC50 outranged the standard ellipticine.

However E. coli K12 strains producing violacein intermediates, showed low or no resistance to predation.

For comparison, the peak intensities of intermediates show a similar change for β-MnO2 and δ-MnO2 at pH 1 and pH 2, respectively (Fig. 4).

Both the on-pathway and off-pathway I120Q intermediates show structure in β1-β3, whish is also strikingly consistent with kinetic folding experiments of αTS.

The foramen structure becomes raised relative to the base layer, and intermediates show an unequal growth around the sides (Fig. 6b and c).

All other significantly populated intermediates show clear lag phases as expected for sequential reactions that are effectively irreversible.

The observation that multiple independent intermediates showed essentially identical kinetics argues against this possibility, but we cannot formally exclude it.

Median vectors (mv representing missing intermediates) show unsampled nodes inferred by MJ network analysis, and the number along the branches shows the position of mutations.

Thus, we propose the formation of the transient pentavalent intermediates shown in steps 3 and 6 of our proposed reaction cycle.