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The identification of cellular substrates revealed that TRiC interacts with folding intermediates of a subset of structurally and functionally diverse polypeptides.
A virtual library of the intermediates of a lipase-catalysed transesterification process was constructed using Chem3D software with p-nitrophenyl acetate as substrate.
These structures, the first for putative reaction intermediates of a type II topoisomerase, suggest how a type II enzyme reseals DNA during its normal reaction cycle and illuminate aspects of drug arrest important for the development of new topoisomerase-targeting therapeutics.
However, the intermediates of a protein unfolding emerge transiently and therefore are hard to study.
In addition, they can sense accumulated non-peptidic pyrophosphate molecules (phosphoantigens), intermediates of a deregulated mevalonate pathway of isoprenoid synthesis, via BTN3A1 (CD277).
Thus we conclude that either these substances are transient intermediates of a pathway needed for the activation of mitochondria, or they are produced in very small amounts and act as signaling molecules.
Similar(53)
The RNA·DNA hybrids fold into structures that are intermediates of A- and B-forms.
The 4 5 mers seem to be structurally dissimilar to 12 18 mers and may represent the intermediates of an alternative, 'off-pathway'.
Extended Data Fig. 3 Stably trapping the acyl-enzyme intermediate of a cysteine protease.
We suggest that the CII and p74 replicons evolved from a stabilized circular intermediate of a progenitor phage related to LE1 [10].
The enzymes encoded by these gene cassettes condense acetyl-CoA with the ß-ketoacyl-S-ACP intermediate of a growing polyketide chain.
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