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A Perl script (see Additional file 3) was run on these sequences to transform them to their inferred original format and all intermediates based on the reaction mechanisms (see Additional file 4).
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All participants were considered to be intermediate and upper-intermediate based on the completion of their previous semesters and the guidelines offered by the institute.
The central ester bond of 2PET was constrained in the oxyanion hole composed of the main chain NH groups of amino acid residues F62 and M131 with the correct orientation to form a tetrahedral intermediate based on the catalytic mechanism of ester hydrolases (Jaeger et al. [1999]).
The C-subunit has been crystallized in several states termed "open," "closed," and "intermediate" based on the relative orientation of the N- and C-lobes.
In Figure 7B we model this pre-activated Hin tetramer intermediate based on the TP901 integrase tetramer (Yuan et al., 2008; Heiss et al., 2011).
In the English breeds Tamworth and Gloucester Old Spots the genetic diversity was low according to the 60K data (He_60K ≤ 0.21) but intermediate based on the NGS data (h_NGS ~ 1.45E-03).
The MICs of orbifloxacin against all mutants were categorised as susceptible or intermediate based on the CLSI breakpoint criteria for orbifloxacin (MIC ≥8 μg/ml) [ 12], except for one strain (strain E9), which exhibited a MIC of 8 μg/ml.
It seems likely that an N intermediate of pR is similarly in equilibrium with its M intermediate, based on the fact that the transient positive (400 nm) absorbance increase is smaller than the negative 500 nm bleach, and the time course at 500 nm shows a partial return to baseline on a timescale of ~50 μs.
The compound 2-naphthol, or β-naphthol, is regarded as the most important chemical intermediate based on naphthalene.
The traditional pattern in the northern Mediterranean basin is an intermediate system based on the integration of forests, tree plantations, and herbaceous crops - with all these resources interlinked by grazing cattle, sheep and goats.
LSM reduces the computational cost by using least squares regression at each intermediate step, based on the results of the following future time step along all the simulation paths (Chow and Regan 2011).
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