Sentence examples for intermediate was assayed from inspiring English sources

Formation of an inactive intermediate was assayed by loss of enzyme activity.

APPBP1-UBA3-catalyzed formation of the thioester-linked Ubc12−NEDD8 covalent intermediate was assayed in 20 μL volumes containing 100 nM of APPBP1-UBA3 or mutants, 3 μM Ubc12, 4 μM wild-type NEDD8 or mutants, and 50 mM Tris-HCl, 50 mM NaCl, 10 mM MgCl2, 5 mM ATP, 1 mM DTT, pH 7.6.

The prodrugs 3a– f and some of the protected hydroxamic intermediates were assayed for in vitro activity against T. b. brucei (Bs427) and in a counter screen for cytotoxicity against a mammalian cell line (HEK 293T).

Cleavage of a half-site by Tyr-324 of Cre, with formation of the Cre-linked DNA intermediate (Figure 2B), was assayed by SDS-polyacrylamide gel electrophoresis (SDS-PAGE).

Potential hydrolysis in a half-site, with either the DNA or the tyrosyl intermediate as target for water attack, was assayed by denaturing urea-polyacrylamide gel electrophoresis (denaturing PAGE).

Following preliminary fractionation of cross-linked amino acids by partition chromatography, the intermediate compounds (DHLNL and HLNL) were assayed by ion-exchange chromatography with post-column derivatization and the mature bonds (PYD and DPD) were quantified using RP-HPLC using their natural fluorescence, as described previously [25].

Intracellular intermediates of glycolysis, tricarboxylic acid (TCA) cycle and nucleotides were assayed as indicators for metabolic changes of central metabolism.

Trace chemicals are assayed.

Plasma hemoglobin levels were assayed.

The Democrats are assaying two certified grownups, Al Gore and Bill Bradley.

"Essaying," Dillon writes, "… is assaying.