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Note that Luc expression depended on VACV infection and co-transfection of the intermediate transcription factor plasmid.
However, an even larger number of early proteins are needed for intermediate transcription than for DNA replication and intermediate expression could be readily demonstrated by transfection of a DNA template.
We found that Luc expression occurred in VACV-infected S2 cells when a plasmid containing the Luc gene under an intermediate promoter was transfected (Fig. 9A), indicating the synthesis of functional viral RNA polymerase and intermediate transcription factors.
These regulatory interactions could be indirect in vivo with other intermediate transcription factors, or direct but with co-factors required.
There is no information on the identity of any intermediate transcription factor but such a factor could also mediate the CEH-14 independent activation of ceh-63.
One possibility is that progerin interacts with an intermediate transcription regulator(s) that regulates a set of genes with certain motifs.
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Considering a certain stimulus as an input signal to the system and mRNA or protein levels as outputs, the connecting network may include interactions between signal transduction intermediates: transcription factors and target genes.
On one hand, the substantial amount of immune-relevant genes involved in metabolic and signalling pathways and the induction of genes encoding cell surface receptors, signalling intermediates, transcription factors, and inflammatory mediators show a clear conservation of mechanisms detected in other vertebrate models, including humans.
This correlation, however, was absent from the ventricle, where intermediate NRSF transcription in LV, RV and Se was accompanied by very low transcription levels of HCN4 (Fig. 3b, c).
The open promoter complex (OC) is a central intermediate during transcription initiation that contains a DNA bubble.
The haplotype variant associated with long lifespan and intermediate Lim3A transcription (GC) was present at high frequency.
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