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We refine the coordinates of the buses so that the coordinates are mapped into the 10 k m×10 k m road map shown in Figure 2. The update period of each bus' location is 1 min, so we complement the hidden intermediate trajectories of buses based on their average velocities.
However, the intermediate trajectories may be longer (curved) in our case.
We used these positions to shift the membrane intermediates in time so that they coincided with the centroids in the average trajectory of GFP-Sla2.
There are three types of transitions between two 3D slides within our 3D document: i) Making an abrupt change from one to another without any intermediate rendered trajectory frames; ii) Interpolation of the viewpoint between the 3D slides in a defined amount of time.
An intermediate evolutionary trajectory may also reflect a cost of lost alleles due to selection caused by the recurrent change of phenotypic optima (see [ 54]).
The design method of the intermediate asteroid flyby trajectory is also considered.
These findings slightly differ from the prospective observational study by Beeckman and colleagues showing that in Belgium only 10-18 % of women had inadequate to intermediate antenatal care trajectory, whereas 64%% had sufficient to appropriate level of care [ 11].
Shown are the measured stabilities for evolutionary intermediates from the trajectory in Figure 2A.
Despite its power to resolve atomic level details, X-ray diffraction captures static structural snapshots; the intermediates along the trajectory of a conformational transition are often lost.
The plausibility of these as distinct intermediates along a trajectory is supported by time-dependent fluorescence studies, which identify at least two distinct ATP-bound states that precede GroES binding (Taniguchi et al., 2004; Cliff et al., 2006).
The code used to generate this figure is in Figure 2 source code 1. DOI: http://dx.doi.org/10.7554/eLife.00631.007 We created plasmids encoding each of the high-confidence protein intermediates along the trajectory, using the same codons found in the natural sequences but not introducing any of the synonymous mutations that occurred during this timeframe.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com