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When we analysed this sequence, the computer told us that all four steps can be combined into one – we made the drug in just one step with double the yield and with no cost of intermediate purification.
The results revealed a high capability of PEG/phosphate systems for the intermediate purification of the highly valuable protein avidin from chicken egg white solutions.
The development and optimization of a purification process of monoclonal antibodies based on two continuous chromatography steps for capture and intermediate purification are presented.
The multimodal resin Capto™ adhere was investigated as an alternative to conventional ion exchange and hydrophobic interaction resins for the intermediate purification of the potential malaria vaccine D1M1.
The steps that are typically involved in purifying proteins can include an initial capture, intermediate purification, and a final polishing step.
These data show that mixed-mode chromatography sorbents, used at capture or intermediate purification steps, really expand the options of MAb purification process development with or without Protein A affinity chromatography.
Similar(32)
Each unit operation has a small number of parameters that configure the behavior of that operation and allow the radiochemist to optimize the synthesis via fine-tuning of heating times and temperatures, stirring, liquid transfer pressures and times, implementation of intermediate purifications, etc.
Lack of such compatible digestion conditions necessitates tedious and time-consuming sequential digestions as well as loss of DNA product during intermediate purifications.
However, the fact that we were able to perform eight chain extension and detritylation cycles, with intermediate purifications, all in the liquid phase demonstrates that it has high potential.
The novel protocol enables the simple and efficient production of 6-[18F]FMT witimet time consuming solvent evaporation and intermediate SPE purification steps.
The eluent from the Δ rimP intermediate affinity purification was first analyzed by negative stain EM.
More suggestions(15)
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