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However, whether the mature insoluble fibril or an oligomeric intermediate play a role in the pathology remains unknown.
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Intriguingly, disruption of verP almost abolished the conidiation of Clonostachys rogersoniana and it was partially restored by addition of the fermentation extract which contains verticillin, implying that verticillin or its intermediate plays a role in the Cordyceps-colonizing fungal morphological differentiation.
Arg273 plays a key role in substrate affinity and His, by acting as a hydrogen bond donator/acceptor in the peptide intermediate, plays a critical role in the enzymatic reaction [ 26].
Nitric oxide and reactive oxygen intermediates play a crucial role in the pathogenesis of myoglobinuric acute renal failure (ARF).
Intermediates play a relevant role in the folding process; many diseases of genetic nature are in fact coupled with protein misfolding, which favours formation of stable inactive intermediate species of a protein.
Alternatively, could intermediates play a role in correct folding, even for proteins that apparently fold efficiently without them?
The C3 (2pyruvatevand and PEP) and C4 (OAA and malate) intermediates play a critical role in the overall network organization.
Nitric oxide, together with reactive oxygen intermediates, play a key role in the activation of disease resistance mechanisms (Delledonne et al. 1998).
Kinetic studies suggested that the formation of the enolate intermediate played an important role in inhibition.
In the electric field, N2H+ intermediate plays an important role, enabling low-temperature ammonia synthesis.
More specifically, sorbic acid has been recently demonstrated to improve the growth of pigs at weaning by regulating IGF gene expression and secretion [ 31], and citric acid, a TCA cycle intermediate, plays an important role as energy substrate for the cell.
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