Sentence examples for intermediate passage from inspiring English sources

Stage 2: intermediate passage through local intermediaries/the domestic market.

We sorted intermediate passage cells based on DNA content by FACS to obtain matched diploid (2N) and polyploid (≥4N) cell populations of the same passage (Figure 2A).

Briefly, intermediate passage (passage 25) cells were stained with 5 ug/ml of Hoechst 33342 (Sigma-Aldrich) for 90 min at 37°C and sorted according to DNA content (2N, 4N, and >4N) using a cell sorter.

Importantly, both the MOSE-I (intermediate passage) and MOSE-L (late passage) cells have also acquired the capacity to form tumors when injected into the peritoneal cavity of syngeneic immunocompetent mice, albeit the former was less invasive [12].

Cells at intermediate passage could be divided into two groups concerning their redox status, as monitored by dichlorofluorescein diacetate (DCFDA) staining.

We compared the transcriptomes of these parasites, grown in intact rats with only a single intermediate passage, with that of cultured monomorphic trypanosomes grown in culture.

Specifically, myogenesis and chondrogenesis showed a moderate decrease over time; osteogenesis was maximum at intermediate passages and adipogenesis was highly sensitive to long-term culture and was diminished at late passages.

Previous studies of undifferentiated female hESCs at intermediate passages have shown three possible states of XCI; 1) cells in a pre-XCI state, 2) cells that already exhibit XCI, or 3) cells that never undergo XCI even upon differentiation.

The heterogeneous MOSE cells undergo distinct phenotypical changes as they are continuously passaged in culture, with early passages representing a premalignant, non-tumorigenic phenotype, intermediate passages representing a transitional phenotype, and later passages progressing to a highly aggressive malignant phenotype when administered to immunocompetent mice.

Rabbits were sometimes used for intermediate passaging of vaccinia virus stocks and for seed virus production, particularly in Europe.

Examination of the four genes implicated in aneuploidy (SCIN, AKAP9, EDN1 and CXCL12) at intermediate passages showed that these mRNA expression changes mostly occur at mid-late passage (P>12) (Supplementary Figure S5A).