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Enzyme inhibitors are often designed to mimic the transition state or intermediate of an enzyme-catalyzed reaction.
It was speculated that hmC could represent an intermediate of an oxidative mC removal pathway.
This fuels the hypothesis that it represents an intermediate of an active demethylation process, which could involve further oxidation of the hydroxymethyl group to a formyl or carboxyl group followed by either deformylation or decarboxylation.
Because dsRNA molecules are known to form as the replicating intermediate of an RNA virus, the dsRNA form of a replicating RNA virus was initially assumed to be the silencing trigger in early models, predicting production of equal amounts of sense and antisense viral siRNAs in infected host cells.
Body mass index (BMI) was excluded in the primary analyses (but included in sensitivity analyses) because it is a potential intermediate of an association between PFCs and asthma.
In contrast, exposure of type 4 CRYs leads to the formation of two-electron-reduced FADH2 (or FADH−) without a detectable intermediate of an FAD− anion radical.
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The RNA·DNA hybrids fold into structures that are intermediates of A- and B-forms.
Extended Data Fig. 3 Stably trapping the acyl-enzyme intermediate of a cysteine protease.
We suggest that the CII and p74 replicons evolved from a stabilized circular intermediate of a progenitor phage related to LE1 [10].
The enzymes encoded by these gene cassettes condense acetyl-CoA with the ß-ketoacyl-S-ACP intermediate of a growing polyketide chain.
Interestingly, 4-hydroxyphenylacetate is an intermediate of a tyrosine degradation pathway found in several Gram-positive bacteria [ 79, 80].
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