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The structure is presented here with covalently bound phosphate in the active site, representing the first phosphoseryl intermediate of a highly active alkaline phosphatase.
Biodiesel is usually produced by transesterification reactions, by intermediate of a catalytic reaction in which a source of triglycerides (from vegetable, animal or residual oils) reacts with excess of alcohol producing as main product biodiesel and as side-product glycerol.
Smart hollow microspheres composed of vinyled-chondroitin sulfate conjugates (CSπ) and magnetite nanoparticles were obtained by the intermediate of a multiple emulsion in absence of a surfactant, attributable to stabilizing properties of the CS.
The proposed mechanism is based on the hypothesis of two-electron oxidation of TMB molecule leading, via intermediate of a radical cation, to the formation of the 2,4,6-trimethoxyphenol (TMP) and an adsorbed polymeric film.
Two potential mechanisms were found as follows: one is a concerted mechanism with a reaction barrier of 18.1 kcal/mol in liquid phase of implicit solvent and 13.8 kcal/mol in liquid phase of explicit solvent of water molecules; the other is a stepwise mechanism with a hydroxylated phosphate reaction intermediate of a quasi-trigonal bipyramid configuration but is less feasible.
By using a combination of kinetic, protein engineering, biophysical and computational techniques we have recently determined the structure of a misfolded intermediate of a PDZ domain and shown that this state is characterized by an alternative packing of the N-terminal β-hairpin onto an otherwise native-like scaffold.
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The RNA·DNA hybrids fold into structures that are intermediates of A- and B-forms.
Enzyme inhibitors are often designed to mimic the transition state or intermediate of an enzyme-catalyzed reaction.
It was speculated that hmC could represent an intermediate of an oxidative mC removal pathway.
In contrast, exposure of type 4 CRYs leads to the formation of two-electron-reduced FADH2 (or FADH−) without a detectable intermediate of an FAD− anion radical.
Body mass index (BMI) was excluded in the primary analyses (but included in sensitivity analyses) because it is a potential intermediate of an association between PFCs and asthma.
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