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An unexpected trans-homodimer intermediate observed in the crystal is confirmed in solution.
The presence and shape of the intermediate observed in the simulations were confirmed by small angle X-ray scattering experiments.
Our results show that the intermediate observed at low concentrations of denaturant has no protection from hydrogen-deuterium exchange, indicating a lack of stable structure.
In all experiments the only intermediate observed in the mackinawite to pyrite reaction was greigite and the data strongly supports the direct transformation of mackinawite to greigite.
The high molecular weight intermediate observed in the LdUba5C217S mutant with anti-LdUba5 antibodies was also evident in the anti-HA immunoblot (Figure 1C).
A paramagnetic intermediate observed in biotin synthase is shown to involve an auxiliary [FeS] cluster whose bridging sulfide is a co-substrate for the final step in the biosynthesis of vitamin B7.
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These mechanisms successfully explain all intermediates observed experimentally.
A stable octahydro-intermediate was observed only with N-heterocycles, whereas a stable hexahydro-intermediate was produced in the polyaromatic hydrocarbon-fluorene. Additionally, the theoretically obtained lowest total enthalpies using DFT calculations agreed well with the stable intermediates observed experimentally in the hydrogenation of fluorene.
Among the intermediates observed in WT E. coli, the class averages present in Group II were the most intriguing.
The endocytic intermediates observed under these conditions, as well as their labeling pattern, were similar to those observed upon high K+ stimulation.
Disparity between concentrations of metabolic intermediates observed after diPAP dosing, compared with FTOH dosing, demonstrates that diPAP exposure does not simply mimic FTOH exposure.
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