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This intermediate methylation level did not translate into a detectable effect on the expression of these genes.
Affected loci, especially in Turner individuals, are characterized by intermediate methylation level in 46,XY males/46,XX females and are typically located outside of CpG islands.
Second, based on its intermediate methylation level and histone modification marks, this site may be linked to regulation of gene expression since it resides within a putative tissue-specific enhancer.
However, analysis of the CpG island with an intermediate methylation level using the four different sized amplicons identified CpG site methylation median SDs of 0.044, 0.040, 0.097 and 0.111, respectively.
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By fragmenting the methylome into bit strings of fixed length of 7 and mapping it to a tone universe of 128 different notes (Fig. 1a), we generate complex musical pieces while still allowing discrimination of potentially important epigenetic control regions, like long stretches of unmethylated DNA versus fully methylated or noisy fragments with intermediate methylation levels.
Differentially methylated CpGs, in most cases, have intermediate methylation levels and tend to occur outside CpG islands, especially in individuals with Turner syndrome.
Additionally, 10 intermediate CpG-density promoters (ICPs) and 7 low CpG-density promoters (LCPs) were found in genistein-mediated DMRs, with the somatic methylation levels of the human counterparts varying from "unmethylated" to "methylated", including four counterparts (Ucp1, Sytl1, Hspb3, and Ripk3) with "intermediate" methylation levels.
A third group had intermediate methylation levels and expressed both proliferative and immune signatures.
The Me value was considered to be useful for analysis of intermediate methylation levels of CGIs.
However, quantitative assessment of intermediate methylation levels has been hampered by the difficulty in appropriate normalization.
All other chord variations with differing durations correspond to intermediate methylation levels.
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