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The subsequent collapse of the intermediate liberates the alcohol (or amine in the case of NGp) from the complex.
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Finally, the benzothiazole N-anion then cyclizes with the acylazolium intermediate liberating the NHC catalyst and producing the desired benzothiazolopyrimidinone 3 in cis configuration.
The end materials ZrMn2, ZrNi2 and the intermediate composition ZrMn1.5Ni0.5 liberate hydrogen only marginally with very poor desorption kinetics.
Thus, the attack of the donor substrate by a nucleophilic Asp residue is predicted to generate a β-maltosyl-enzyme intermediate that is subsequently liberated by an acceptor.
Sporulated oocysts can then be ingested by intermediate hosts, where infectious sporozoites liberated from oocysts differentiate into tachyzoites completing the life cycle [ 7, 11].
A (Z -enol ether bridge of 231 reacts with O2 to give the dioxetane intermediate 232, which cleaves to liberate the photosensitiZ -enolthetherbridge in the profimity of a tumor cell.
A comparison of the size and nature of the target mineral for flotation (pyrite) in the concentrates from both these unit operations has shown that they both recover predominantly liberated pyrite in the fine and intermediate size classes (−150 μm).
The resulting intermediate plasmid was then digested with XbaI to liberate the yeast helper fragment, and the backbone was religated to yield pCRISPomyces-1.
When the eggs are discharged in water they liberate the larvae (miracidium) which infect the intermediate host (some species of aquatic snails) where they mature to secondary larvae (cercariae).
The N-terminal membrane-attached intermediate is further cleaved by Site-2 protease (S2P), liberating the transcription factor from the membrane.
Nuclear cleavage of the pri-miRNA by Drosha, a RNase III-type enzyme, liberates an ∼60- to 70-nucleotide stem loop intermediate known as the miRNA precursor (pre-miRNA; Filippov et al. 2000; Snyder et al. 2009).
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