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Briefly, the samples were differentially isotopically labeled in parallel by adding light or intermediate isotopic formaldehyde and NaBH3CN to the reaction.
In the case of Lake Nakuru, even a mixed rotifer sample would have led to intermediate isotopic values (δN: 9.2‰; δ C: −17.2‰) and to substantially different outputs from isotope mixing models.
Synthetic peptides and an islet tryptic peptides mixture were dimethyl labeled in solution with reagents containing light and intermediate isotopic formaldehyde, respectively, as described by Paul Boersemaa et al. 2009).
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Now, F2 gas can be considered as a necessary intermediate in uranium isotopic enrichment.
All the glycolytic intermediates reached an isotopic steady state after about 15 min (< 5% variation from the enrichment at 4 h).
However, the C incorporation into the individual intermediates did not approach isotopic steady state for at least 30 h.
The C incorporation into the individual intermediates did not approach isotopic steady state for at least 30 h, thus requiring non-steady-state approaches for flux analysis.
Cells were harvested 24 h after labeling with [C]/[C]glucose, and the polar metabolome was extracted as previously described and analyzed by SRM-based targeted LC MS/MS for both nonisotopic and isotopic glycolytic intermediates.
It measures protons to 150 MeV and has isotopic resolution for intermediate mass nuclei.
In spring, blood isotopic composition exhibited intermediate values between those of summer and autumn, falling between the expected predicted values for a pure-invertebrate and a pure-bird diet.
Hickey-Vargas et al. (2008) analyzed the two gabbros and concluded that they have an Indian MORB Hf-Nd isotopic signature, with Pb isotope ratios intermediate but closer to Pacific MORBs than the WPB, which has a strong Indian Pb isotope signature.
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