In other words, for certain combinations of m and r, the average invasion probability as a function of qc is maximized at an intermediate (nonzero) value of qc.
Group 1 members SmVAL2, SmVAL3, SmVAL5 and SmVAL9 all share a similar expression pattern with transcription peaking in miracidia, suggesting roles for these gene products in the free-living stage or in intermediate host invasion.
In bacterial recombinational repair, the strand invasion intermediate is generated by the RecBCD helicase/nuclease in a complicated reaction requiring 5' to 3' helicase, 3' to 5' helicase, 5' to 3' nuclease, 3' to 5' nuclease, and a specific DNA sequence called chi.
Structure-selective nucleases could cleave the strand invasion intermediate resulting in several possible outcomes.
We suggest these events are due to cleavage of the strand invasion intermediate, transferring the DSB from the linear CFV to the chromosome to initiate secondary BIR events, further increasing the genomic instability associated with this mutagenic DNA repair mechanism.
The Mus81-Mms4 (Mus81-Eme1 in fission yeast) heterodimeric nuclease resolves strand invasion intermediates by two sequential cleavages of branched intermediates to form crossover products.
The advantage of both systems is that 50 100% of the target sequences are cut within 1 hr following induction of the nuclease, and, by taking DNA samples at different times after DSB formation, intermediates in the process, for example, resected DNA ends and strand invasion intermediates, can be identified by Southern blot hybridization and/or PCR methods.
RECQ1 may utilize its motor ATPase function to displace Rad51 from inappropriate strand invasion intermediates that are either homeologous or occur in an untimely manner.
The 3′ tails are acted upon by strand exchange enzymes to form single-end invasion intermediates (SEIs).
Although in vitro studies have revealed differences between the biochemical activities of RTEL1 and Srs2, both proteins counteract HR by dismantling DNA strand invasion intermediates.
