Sentence examples for intermediate gene expression from inspiring English sources
During the first, early transcriptional phase, factors are expressed that are involved in viral DNA synthesis, intermediate gene expression, and modulation of the host anti-viral response [11].
Thus, the early proteins needed for intermediate gene expression were synthesized and the data strongly suggested a block in viral genome replication in S2 cells.
Nevertheless, synthesis of the multitude of early proteins needed for intermediate gene expression was demonstrated by transfection of a plasmid containing a reporter gene regulated by an intermediate promoter.
The DNA replication requirement for intermediate gene expression in mammalian cells can be overcome by DNA transfection [38], since the viral transcription factors needed are early gene products [39].
The question of whether a host inhibitory factor is present or a host stimulatory factor is missing might be answered by carrying out a comprehensive siRNA screen to test the former and a forward genetic approach by transfecting a human cDNA library into S2 cells to test the latter, in each case using plasmid replication or intermediate gene expression as the read out.
There is also evidence that p38 MAPK is able to drive MHC type IIx (intermediate) gene expression in myoblasts [ 26].
Low levels of BMP activate intermediate mesoderm gene expression, whereas high levels of BMP repress intermediate mesoderm gene expression and activates lateral plate mesoderm genes [14].
The intermediate EpiSC gene expression level in early EECs supports this point.
To determine the impact of the accumulation of DNA repair intermediates on gene expression, the five yeast strains with altered expression of BER repair enzymes were analyzed for differential gene expression.
While the RNA-guided nuclease Cas9 has been repurposed as a programmable transcription regulator, its application has typically been limited to binary on/off regulation and thus misses informative and potentially optimal intermediate levels of gene expression.
However, by transfecting plasmid templates, we bypassed the requirement for replicated viral genomic DNA and succeeded in demonstrating intermediate and late gene expression.
