Attempts to show colocalization of Btn1p at pH 4.0 with standard organellar and typical vesicular trafficking intermediates failed to identify these puncta.
Work with isoprenoid analogs incorporating electron withdrawing fluorine substituents (4) provides evidence that the transition state involves some carbocationic character although analogs including 5 designed to trap such intermediates failed to do so.
OCRL1 lacking the LIDLE clathrin-binding site, which is required for OCRL1 incorporation into clathrin-coated trafficking intermediates (15), fails to rescue apoptosis in the mutant.
In [11], a multipath scheme between sender and receiver allows the TCP session to be rerouted when intermediate nodes fail.
Moreover, H5 HAs bearing intermediate sequences failed to bind airway tissues and likely represent mutations that are an evolutionary "dead end".
It is also noteworthy, albeit perhaps not surprising, that the intermediate group failed to produce a significant enrichment profile and that differences are only visible when comparing the extremes.
Though the study by Oostendorp found a significant difference between treatment groups at 12 weeks' follow-up (intermediate), it failed to show differences at six weeks and 24 weeks.
Exploration of alternative pathways with greater complexity, including reversible activation and mixed intermediate species, failed to yield improved fits; similarly, removal of the initial unimolecular step resulted in a much poorer correspondence with the data.
The loss of Tbr2, a nuclear regulator of intermediate neuronal progenitors, fails to generate postnatal DG (Hodge et al., 2012) and Tbr2 mutants have postnatal abnormalities of the DG similar to the DG of Smo mutants (Machold et al., 2003).
Previous analysis performed on CD14HIGH bright and CD14LOW monocytes in patients undergoing cardiac surgery with cardiopulmonary bypass, or following low to intermediate risk surgery failed to detect a differential downregulation of HLA-DR on monocytes subsets [ 42, 43].
