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Solving (25) gives the following intermediate expressions for tag response: (26).
Although so far, we have only used N-best lists as intermediate expressions for our integrative method, other expressions with less information loss could also be used, such as word graphs or HMM trellises.
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Strong expression of erbB2 was showed for the case code 1 patient (score +++), with intermediate expression of erbB2 for the case code 2 patient (score ++) and low expression for the case code 3 patient (score +) (the Additional file 5: Supplemental Figure S2A).
Staining of the cells with a CXCR4-specific antibody and FITC-labeled secondary antibody followed by FACS analysis of positive cells revealed the highest CXCR4 expression in Pat-1, Pat-3 and Pat-8 cells, whereas all other patient-derived specimens had an intermediate expression of CXCR4, except for Pat-5 cells, in which CXCR4 expression was not detectable.
pDCs, which were identified by their intermediate expression of CD11c and high expression of CD45RA, were uniformly very low for Id2-GFP (Supplementary Figure S1).
Clone #2, with intermediate expression of hPARP-1, was designated PARP-WT and used as a positive control for subsequent experiments.
Group 5 showed intermediate expression of most traits (Table 3).
Cells with intermediate expression of CD16 were designated as CD16int.
The switch-like behavior is seen in the second histogram, where the frequency for intermediate expressions is very low, but higher frequency is observed at the extremes (close to 0 or 1 fractional expression).
For T. delaisi, differential expression was found for sstr1, which was not measured in cichlids, with significant differential expression between territorial males and females (and intermediate expression levels for the sneaker male).
The remaining component of the CV is given by the standard deviation of expression, which has a negative quadratic dependence on the mean of expression (Additional file 3: Figure S1) showing higher values for intermediate expression classes.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com