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In all tissues, a near bell-shaped curve was observed, where genes with intermediate expression levels were higher in 5mC than weakly or highly expressed genes.
The correlations show a similar pattern (with a Spearman correlation of 0.54) to previous reports comparing results of microarrays and other DGE data (t Hoen et al. 2008; Morrissy et al. 2009) or RNA-Seq data (Wang et al. 2009), where genes at intermediate expression levels are more correlated between platforms than very high- or low-expressed genes.
The EGFR-cocluster class showed intermediate expression levels of Proneural and Mesenchymal signature genes.
Nine of 21 lSSc-NoPAH samples clustered on the same branch and showed intermediate expression levels.
A striking feature of the gene expression is a gradient with the largest differences between the lSSc-PAH and controls, with lSSc-NoPAH showing intermediate expression levels.
With an absolute measurement of Bicoid concentration – the equivalent of which did not exist in case of Elowitz et al [1] – the theory predicts that we should see a non-negligible contribution to the noise peaking at intermediate expression levels.
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However, at the intermediate expression level (2 5 WTU) the Z-ring may constantly transition between the ring-like and compressed helical conformations, whereas at high expression level (>8 WTU) the Z-ring predominately exists as helical structures that are visible to conventional FLM, similar to previous observations when FtsZ was highly overexpressed [8], [13].
Hence a key signature of input noise is a peak at some intermediate expression level as shown in Fig. 2. The claim that many genes have expression noise levels which fit the universal output noise model of Eq (1) would seem to contradict the prediction of a peak in the noise as a function of the mean.
Lines L6 and L8 showed intermediate expression level compared to lines L1, L2, L4 and L11.
An intermediate expression level was seen in vulva, trachea, kidney, colon, ovary, cervix, small intestine and pancreas and, finally, lowest expression was observed in bladder, lung, stomach and rectum.
These single-cell data suggest that the intermediate expression level of the csgB gene at population level corresponds to a heterogeneous mix of curli-on and curli-off cells in the bacterial population.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com