The YFP+ cells show an intermediate expression of CD11c, the classical marker expressed on dendritic cells (DCs).
This may be due to DNA methylation's tendency to be most highly targeted to genes of intermediate expression, while H3K9ac is known to target very highly expressed genes.
In all tissues, a near bell-shaped curve was observed, where genes with intermediate expression levels were higher in 5mC than weakly or highly expressed genes.
gbM is found in plant genes with intermediate expression level, which evolve slowly, and is often broadly conserved across millions of years of evolution.
Additionally, we found gbM genes to be significantly longer than unmethylated genes (Table 1) and to have intermediate expression levels (supplementary Fig. S1).
Integrin beta-1 expression showed a different pattern, with comparable expression levels on PBMC and granulocytes, intermediate expression on platelets and very high expression on both B2M KO and wild-type MDBK cells.
Another concern is the imbalanced group sizes; however, a reduced analysis with an equal number of samples per group reiterated our findings that NAT displays an intermediate expression state between healthy and tumor (Supplementary Fig. 6).
In plants, gbM is usually found in genes with intermediate expression level, which evolve slowly, and is often broadly conserved over millions of years of evolution (Zhang et al. 2006; Takuno and Gaut 2012, 2013; Bewick and Schmitz 2017).
For instance, as previously reported (Takuno and Gaut 2012, 2013; Bewick and Schmitz 2017), gbM genes were significantly longer than unmethylated genes and had an intermediate expression level (Table 1 and supplementary Fig. S1).
Solving for yields the intermediate expression (16).
Group 5 showed intermediate expression of most traits (Table 3).
