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BMC described a sigmoid curve, with an initial phase of rapid increase from 0.285 ± 0.075 g (5 weeks) to 0.394 ± 0.025 g (9 weeks of age) (P < 0.001), an intermediate exponential phase from 9 to 21 weeks (P < 0.01), and a subsequent stationary phase.
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Strains were grown in YPD medium to exponential phase.
Switching to fresh medium (controls) in late exponential phase stimulated growth, and switching to N-free medium (N deprivation, -N) induced an intermediate response; slightly lower growth than in the controls, but higher than that of the standard continuous cultures.
Based on these facts, expression induction is done when culture is at the exponential phase, but depending on the induction system and the recombinant protein, expression could be done at the initial, intermediate or final part of that phase.
Exponential phase cells were used for further experiments.
Kinetic folding of this domain showed multiple exponential phases, whereas unfolding showed a single exponential phase.
Culture media was changed per 48 h, and exponential phase cells were used for experiments.
The cycle threshold (Ct) is set within the exponential phase of PCR.
Growth curves were established for each microbe 1 4 days to determine the log (exponential phase).
When the cells reached exponential phase, resveratrol was added to 20 mL of diluted culture.
Over the past seven years, NEWA's growth has hit the exponential phase!
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