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A small number of resonances disappeared or broadened significantly during the titration, indicating an intermediate exchange rate.
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The air exchange rates during the experiments were either high (working hours) or low (non-working hours) and ranged from 1.6 to>12 h−1, with intermediate exchange rates.
This property, together with the previously noted large errors in the rate constants for fast (k1) and intermediate (k2) exchange rate constants in Bs-DHFR, led us to focus on the plateau regions of the time courses, which reflect the very slow, effectively nonexchanging, amide hydrogens (k3 < 0.002 min–1) on the time scale of the experiments (300 min).
Removal of both ZnII ions from NDM-1* using 10 m M EDTA (ethylenediamine tetraacetate), led to the observation of broader signals reflecting at least two conformers of apo-NDM-1* in a intermediate exchange system (exchange rates are considered relative to NMR chemical shift time-scales[ 13] throughout), as indicated by significant line broadening.
Thus, if the diffusive exchange is diffusion limited for the drug molecules in the Rf core/IPDU intermediate-layer region, the exchange rate constants are small.
The intracellular environment often affects dynamic processes such as chemical reactions or conformational exchange on the μs to ms time scale, and exchange rates can experience modulations toward slower, more unfavorable NMR time-scales (i.e., intermediate exchange).
Exchange rate.
The exchange rate.
The exchange rate may help.
The exchange rate is trickier.
The exchange rate helps.
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