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We identified variants in the CC domain with intermediate effects in transcription that correlate with failure to interact with the PALB2 protein (Figure 3).
Moreover, the extent of upfield proline chemical shifts correlated with aromatic electronics, with the largest effects in the X = tryptophan peptide, the smallest effects in the X = pentafluorophenylalanine peptide, and intermediate effects in peptides with other aromatic amino acids.
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Kindling-induced granule cell axon sprouting as measured by the Timm stain was also attenuated in homozygous null mutants compared with +/+ mice, with an intermediate effect in +/- mice.
However, RBV had a surprisingly mild effect on the VSV-driven GFP expression in BHK21, Vero and A549 cells even when used at 1000 µM concentration with a somewhat intermediate effect in 4T1 cells (Fig. 1B).
Importantly, a strong correlation between BPTF levels and the growth inhibition effect was noticed as the most pronounce effect in growth inhibition was witnessed in WI-38HP-2 while an intermediate effect in WI-38THP-1 and a mild effect in WI-38T were witnessed, indicating that BPTF is one of the major players that contribute to the pre-malignant phenotypes observed.
The 12 × 3 treatment had an intermediate effect in B6.
The application of nigericin inhibited qE similarly in adg1-1/tpt-2 and the wild type, whereas the application of DTT had strong inhibitory effects on qE in adg1-1/tpt-2 and an intermediate effect in the wild type.
Interestingly, the same experiment was also repeated for the Flag-TDP-12xQ/N ▵CRM1/2, ▵constructct, and that in the experiments presented in Figure 4 showed an intermediate effect in affecting the splicing profiles (both CFTR reporter and endogenous POLDIP3).
Addition of the potent destabilizing residue, Phe, to LF resulted in significantly decreased LF potency for both macrophages and rats, while an intermediate destabilizing residue, Arg, showed predictable intermediate effects both in vitro and in vivo.
However, the intermediate effects seen in the 'reduced muscle' phenotype (Myf5 nlacZ/+:Myod −/−), indicate that the effects on the mutant limbs are unlikely to be from trophic factors from the muscle alone.
These observations suggest that differences in intermediate effects must account for the difference in sensitivity of the proliferative response to the xenoestrogen.
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