Sentence examples for intermediate constructs from inspiring English sources
Cloning strategies that require many successive steps can now by done in two steps: one being preparation of the intermediate constructs and the second, assembly of the final construct.
This is expected because shuffling is performed without the use of PCR; the modules in the intermediate constructs are made using PCR but are then sequenced before being used for restriction-ligation assembly.
In addition, because of the restriction-ligation, only the desired assembled products are expected to accumulate since all other ligation products (for example, ligated products containing plasmid backbone DNA from the intermediate constructs) will contain BsaI sites and should therefore be immediately redigested with BsaI.
Endophenotypes refer to quantifiable intermediate constructs that index an underlying liability to a disorder or behavioural trait.
The present approach is to define SES variables and their potential impact on childhood obesity rates in terms of three critical intermediate constructs: money, place, and time.
Step iii) the replacement of the rpsL-neo selection cassettes from the intermediate constructs using linear DNA fragments was achieved through counter-selection and Red/ET recombination.
It is shown that the correlation functions can be expressed in terms of an intermediate construct, called the texture function; the correlation functions have quadratic dependence in the texture functions.
INTERMEDIATE construct (figure 3B and C).
This intermediate construct was named pEGFP-C1/JV 5' GS.
The TMV-based vector was made in several cloning steps with intermediate construct (IC) formation.
The use of a pELB-n end-linker rather than a basic end-linker in the first round of assembly results into a level 2i (intermediate) construct (Level 2i-1; Fig. 4A), which contains, in addition to the cloned transcription units, two BsaI restriction sites for a next round of cloning and a lacZα fragment as selectable marker.
