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The motor for retrograde IFT is cytoplasmic dynein 2, which in almost all organisms investigated contains dynein heavy chains, intermediate chains, and light-intermediate chains that are specific to this dynein, as well as light chains that are shared with other oligomeric dyneins.
Cytoplasmic dynein is a large protein complex (~1.5 MDa) containing heavy chains, intermediate chains, light intermediate chains, and light chains (Pfister et al., 2005).
SSIIai played a major role, while SBEIIbi played a minor role in accumulating intermediate chains (DP12 ~ 22).
The variations in short and intermediate chains were related with starch pasting and thermal property changes (Park et al. 2007).
The SSIIaj allele starch showing more short chains and less intermediate chains was determined with a remarkably high RCL10/24.
Two genes (Dync1i1 and Dync1i2) in rodents encode cytoplasmic dynein intermediate chains, in which Dync1i1 is a neuron-specific gene (Fig. 1A).
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We therefore prepared liposomes with lipids of intermediate chain length, using dimyristoyl phosphatidylcholine containing 14 carbon chains.
Both dynein intermediate chain and p150(Glued) also became hyperphosphorylated during M phase in XTC-2 cells and HeLa cells.
We partially purified p83, obtained peptide sequence, and identified it as the intermediate chain of cytoplasmic dynein.
147, 481 491 (1999).Demonstration that pericentrin interacts directly with a component of cytoplasmic dynein, the light intermediate chain (LIC), suggesting a role for LIC in centrosome assembly.
Purohit, A., Tynan, S. H., Vallee, R. & Doxsey, S. J. Direct interaction of pericentrin with cytoplasmic dynein light intermediate chain contributes to mitotic spindle organization.
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