This gap, which constituted the ground upon which modern infantrymen found themselves fighting, had to be filled by another new weapon, which would fire a cartridge of intermediate power.
An intermediate flush of the cartridge with salt-free water/acetonitrile removed large amounts of phosphate-free contaminants, a step similar to a procedure described earlier, in which, however, triethylamine buffer was used as "ion pair" reagent.
A new GAM module design allowed for the connection of modules in series, intermediate data sampling points, and removable membrane cartridges.
Most GPMGs were chambered for the intermediate-size 7.62-millimetre cartridges of NATO and the Soviet Union, while SAWs fired small-calibre, high-velocity rounds such as the 5.56-millimetre NATO or the 5.45-millimetre Kalashnikov.
The HLB cartridge was eluted with ethanol into the intermediate vial, which contained a low pH HPLC buffer.
Peptides were labeled on SepPak C18 cartridges (Waters) with labeling reagent (light and intermediate, with CH2O (Fisher) plus NaBH3CN (Fluka) or CD2O (Isotec) plus NaBH3CN, respectively) as described in Boersema et al (2009).
The intermediate was collected and passed through a pre-conditioned C18 cartridge to concentrate the radioactivity, which was then eluted with ether and passed through a dry cartridge to the second reaction vessel for the deprotection step.
Cartridges can be plumbed between reaction vessels for intermediate purification or for final preparation of probes that do not require high-performance liquid chromatography (HPLC) purification.
After the initial radiolabeling step and prior to deprotection, the intermediate was isolated and eluted by ether and passage through a Dry Sodium Sulfate Plus Long cartridge to remove residual moisture.
The intermediate is dissolved in an excess of water and transferred to an Oasis HLB Plus-cartridge.
