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Cytoplasmic dynein consists of heavy, light, intermediate, and intermediate light chains in addition to several regulatory subunits [ 33].
Dyneins are multi-subunit protein complexes with two or three heavy chains (DHCs), light chains, light intermediate, and intermediate chains [ 19].
Namely, µI is varied around the by θ*, considering that θ* is a variable whereas θI is not an independent variable because of θI+ θ*=1 in our two-step model (but generally, θI+ θ*≍1 if there are more than one intermediate and intermediate I is the main one).
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They conducted a study with 206 students divided into two levels of language proficiency (pre-intermediate and intermediate), on one hand, and into two experimental groups and one comparison group, on the other.
Particularly for CpG sites with low-intermediate and intermediate levels of methylation, we found an even higher mutation rate with higher coverage.
Finally, the CpG sites in intergenic regions and intronic regions showed a higher mutation rate with low-intermediate and intermediate levels of methylation.
Within chromosomes, we observed that CpG sites with low-intermediate and intermediate levels of methylation had a higher mutation rate in all autosomes compared with that of other methylated CpG sites.
This finding suggests that our observation that CpG sites with low-intermediate and intermediate levels of methylation actually had a much higher mutation rate is not affected by the sequence coverage of methylated sites.
Within autosomal chromosomes, we observed that CpG sites with low-intermediate and intermediate levels of methylation had a higher mutation rate compared with CpG sites with other methylation levels.
Altogether, the results in Figure 1 are consistent with a model in which the VSD undergoes two sequential transitions, resting-to-intermediate and intermediate-to-activated. The first transition is sufficient for KCNQ1 to open, resulting in both intermediate-open and activated-open states.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com