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A great deal of sculpture is designed to be placed in public squares, gardens, parks, and similar open places or in interior positions where it is isolated in space and can be viewed from all directions.
To investigate the interplay among protein folding kinetics, thermodynamics, and pKa modulation, we introduced a pair of Asp residues at neighboring interior positions of a coiled-coil.
We quantified within-clutch size variation at hatching and found that newly hatched larvae from the periphery (5 mm from edge) of 2-dimensional clutches were smaller in standard length, cranial depth, eye diameter and body area (7%, 8%, 4% and 11%, respectively) than larvae from the interior positions within clutches.
Based on its interior position and high level of connectedness, it is likely to be ancestral.
Early work in this area showed that natural haemoglobin variants tolerated substitutions at exterior positions much more readily than interior positions [22], [23].
Upon introduction of an extra copy of Chromosome 7, we observed a tendency for CT-18 and CT-19 to be shifted to a more interior position (ΔM = −5.59 and ΔM = −3.02, respectively).
It is well-known that proline residues disfavor interior positions in α-helices; in the referenced study, the authors were not able to identify any examples of α-helices containing proline at an interior position.
Phosphorylation was more disruptive to the α-helix at a more C-terminal interior position (residue 10) than at a more N-terminal interior position (residue 5) of the α-helix.
Genealogical networks showed that these shared alleles are located at central interior positions, indicating that they are ancient alleles (fig. 1).
