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Any curfew breaches or interference with the tag is reported to a control centre.
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This tether length was chosen as a trade-off between the need for the tag to have a good chance of breaking the sea surface to transmit in air when the sunfish was near the surface, and the need to minimise drag to the fish and any interference of the tag with fin movements during swimming.
Although it was previously shown in neuronal cells that GFP Dpl behaves like the wild-type protein [ 24], in order to discard a possible interference of the tag with the metabolism of native Dpl in our cell system, we stably transfected FRT clones with native Dpl alone, or together with PrPC, and repeated all the experiments shown for GFP Dpl in these clones (see the Experimental section).
An interference with the sub-cellular localisation of tagged proteins is more difficult to detect as addition of N- or C-terminal tags is known to sometimes interfere with the localisation of the fusion protein [37].
The proteins were tagged at their C-termini to avoid interference with the N-terminal mitochondrial targeting sequences.
We then constructed a compound tag with the tag name and its frequency.
Create the HTML form with the tag.
Close off the table with the tag.
This data suggests that in agreement with work published by Xu and co-workers[37] the affinity tag should be placed at the N-terminus of the recombinant NA to avoid interference with the formation of functional homotetramers.
In the second labeling strategy, we introduced the TC tag into flexible regions of the RT and Env in order to limit the structural and functional interference with the protein.
We had enough of interference with the Baath Party".
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