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Transfections of expression vectors or RNA interference were performed as previously reported [44], using Lipofectamine (Invitrogen).
Screening of synovial biomarkers of inflammation and testing for HA interference were performed on explant supernatants from eight study participants.
Further experiments with RNA interference were performed to study whether IL-27 exerts its effect in humans by modulating IRF8.
Following phylogenetic and transcription factor binding profiling, electrophoretic mobility shift assay (EMSA) and RNA interference were performed and the effects of incubation with mithramycin, an antibiotic that binds GC boxes, were also studied.
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In the proposed scheme, based on the superposition probabilities obtained in Eq. (13), the reliable detection of each interference is performed.
A discussion of the proposed method, as well as analysis of its accuracy and susceptibility to external interference is performed, in addition to its validation in real substations, so as to prove the efficiency and applicability of the proposed method.
After all, who doesn't find the idea of lazing about in a Parisian park clutching a petite bier while "Interference" is performed at brain-splitting volume?
In order to answer this question, siRNA interference was performed to silence TSP-1 expression.
RNA interference was performed with a "Smart Pool" mixture targeting human TBCD (Dharmacon, CO, USA).
RNA interference was performed as previously described.
Interference was performed as described previously [ 83].
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