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In conclusion, we developed a specific ELISA method using the bioactive region in the dTMP-GH as the immunogen, which could eliminate the interference of endogenous rGH on dTMP-GH detection.
Thus, using the polyclonal antibody as the capture antibody, we can ensure that the dTMP-GH fusion protein with platelet activating activity is captured, and the interference of endogenous hGH is excluded.
To prevent interference of endogenous RhoA in our experiments, we designed an adenovirally delivered miRNA construct targeted against a conserved region of RhoA, as shown in Figure 3B.
There was no interference of endogenous compounds in the chromatographic determinations.
Therefore, we could ignore the interference of endogenous activin A and TGF-β1.
ADA was added to the assay to eliminate the interference of endogenous adenosine in the basal [S]GTPγS binding.
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The validation of an immunoassay to quantify plantibodies is a relatively complex task due to the complexity of the plant extract, the low level of expression of this molecule, and the potential interferences of endogenous peroxidases contributed by plants.
The selectivity of the method was determined by six different rat blank plasma samples, which were pretreated and analyzed to test the potential interferences of endogenous compounds co-eluting with analyte and IS.
Also, enforced HOPX expression inhibited tumor growth and RNA interference knockdown of endogenous HOPX restored it [ 7, 8, 15].
To rule out the possibility that this transcriptional regulation resulted from the interference of the endogenous p53 by the p53-expressing adenovirus, we also tested the promoter activity in human colon cell line HCT116p53+/+ and HCT116p53-/ cells.
To localize the site(s) of phosphorylation, the effect of 200 mM sorbitol was measured on truncated forms of AdcA, AdcAΔHΦGFP and the HΦGFP expressed in both a KAx-3 and adcA null background to exclude a possible interference of the endogenous AdcA.
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