For a Linnik interference microscope objective, the reference objective and the reference mirror are moved together to match the path lengths in the reference and test arms to maximize fringe contrast.
Images were obtained using a 63×/1.4 Plan-APOChromat differential interference contrast objective or a 40×/1.3 Plan-Neofluor oil objective.
Given the total emission power P and the nearby networks interference, the objective is to generate baseband pulse entirely matching the target spectrum G(f).
with a Plan-Apochromat 63 × NA 1.4 oil differential interference contrast objective lens.
All experiments were done with a 60 × oil differential interference contrast objective lens.
A Plan-Apochromat 63×/1.4 oil DIC (differential interference contrast) objective was used and a frame size of 488 pixels×488 pixels×1 pixels.
To study cell shape, hESC-PLTs were fixed with 1% glutaraldehyde in platelet buffer for 20 min, moved to a coverslip chamber, and viewed with a 63× differential interference contrast objective.
Transfected cells were perfused with standard recording solution (as for the electrophysiology experiments) at 30 32 °C, and imaged with a Zeiss Axioskop LSM510 confocal microscope equipped with an Achroplan ×40 water immersion differential interference contrast objective (numerical aperture, 0.8).
Samples were visualized by confocal microscopy using a Zeiss LSM 510 meta with a Plan-Apochromat 63×/1.4 Oil differential interference contrast objective lens and images acquired using LSM 510 version 3.2 software (Carl Zeiss, Jena, Germany).
Cells were examined with an Eclipse E600FN microscope (Nikon Instruments, Sesto Fiorentino, Italy), equipped with a water immersion differential interference contrast objective and an infrared CCD 100 camera (DAGE-MTI, Inc., Michigan City, IN, USA).
