Chromatography was performed on a Surveyor autosampler/LC system (Thermo) and 0.3 x 150 mm PLRP-S column (3 μm, 100 Å, Agilent) interfaced with the mass spectrometer by an Advance captive spray source (Michrom).
Both single dimensional high pressure liquid chromatography (LC) and multidimensional LC (LC/LC) can be directly interfaced with the mass spectrometer to allow for automated collection of tremendous quantities of data.
Chromatography was performed on an Easy-nLC II (Thermo) at 40 ° C, using a 0.1 X 300 mm Magic 3 μm, 200 Å C18AQ column (Michrom Bioresources, Auburn, CA, USA) interfaced with the mass spectrometer by an Advance captive spray source (Michrom Bioresources).
Additionally, the chromatographic system was coupled with a mass spectrometer (MSn) equipped with an ESI interface and operated with the Mass Hunter software.
Rather, the helix-loop-helix motif in our structure forms a relatively flat surface and thus a more intimate interface, with the centers of mass of the two hemicapsids moved closer together than has been proposed for MSV.
The TMT-labeled products were separated by a 65-min gradient elution at a flow rate 0.25 μl/min with the EASY-nLCII- integrated nano-HPLC system (Proxeon, Denmark), which is directly interfaced with the Thermo LTQ-Orbitrap mass spectrometer.
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Further improvements in sensitivity and detection limits can undoubtedly be implemented by mating the interface with a mass spectrometer equipped with a more advanced ion optics, mass analyzer, and multichannel plate ion detector.
This work reveals a new physical property of FSI problems involving thin interfaces with mass: the inertia of the thin fluid structure interface regularizes solutions to the full FSI problem.
The chromatographic system was coupled with a mass spectrometer (MSn) equipped with an ESI interface and operated the Mass Hunter software.
