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Considering the supplementary data set of Kim et al. for singlish interface hub proteins (Kim et al. 2006), we obtained similar result (table 2).
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Moreover, singlish interface hubs evolve faster than multi-interface hubs but are generally slow evolving than rest of the proteome due to their hub nature (Kim et al. 2006; Clarke et al. 2012).
Proteins supplementary data of Kim et al. (2006) was used to obtain structurally resolved singlish interaction interface hubs (≥ 5 interaction partners + at most 2 interaction interfaces).
The affinity of interactions between the proteins of the multi-interface hub tended to be higher than that between the proteins of the single-interface hub [4].
In nature, however, it is possible that a multi-interface hub transiently interacts with its individual partner through the corresponding binding interface at different spatial-temporal conditions.
For example, although a multi-interface hub is more likely to form a permanent complex with its partners through permanent interfaces, it does not rule out the possibility that the multi-interface hub forms a transient complex with its partners through transient interfaces.
Finally, we established that ePPID could predict protein evolutionary rate better than the number of distinct binding interfaces for hub proteins in the Structural Interaction Network and clarified the slower evolution of co-expressed multi-interface hub proteins over that of other hub proteins.
Moreover, the application on hub proteins in the Structural Interaction Network provides further support that ePPID also gives a better prediction of protein evolutionary rate than the number of distinct binding interfaces and clarified the slower evolution of co-expressed multi-interface hub proteins over that of other hub proteins.
Analysis of hub proteins in the Structural Interaction Network further supported ePPID as a better predictor of protein evolutionary rate than the number of distinct binding interfaces and clarified the slower evolution of co-expressed multi-interface hub proteins over that of other hub proteins.
It was found that multi-interface hubs are mostly party hubs and singlish-interface hubs are mostly date hubs [ 22].
We found that non-co-expressed singlish-interface hubs, non-co-expressed multi-interface hubs and co-expressed singlish-interface hubs evolve at a similar rate.
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