Exact(15)
Some of the more interesting transcripts that were regulated in common were one general transcription factor (related to tfbE), a cell wall/membrane, ABC-transporters, and one replication origin recognition (orc/ cdc6).
These included gene fusions, gene deletions, rare or aberrant transcriptional isoforms, ncRNAs, and transcripts of unknown function (TUF); a subset of interesting transcripts was validated using RT-PCR on the RNA obtained from the original breast cancer sample as well as from other eight carcinomas with the same histotype.
The expression levels of interesting transcripts were normalized to the housekeeping β2 microglobulin (B2M) and PPIA gene transcripts.
When the long HML8 ORFs were used in a search against RefSeq cDNA sequences, several interesting transcripts were found: i.
While this step reduces the number of false positives, it also may eliminate interesting transcripts.
The expression levels of interesting transcripts were normalized to the housekeeping TATA-box binding protein (TBP) gene transcripts.
Similar(44)
The gene involved in HD, interesting transcript 15 (IT15), encodes for the protein huntingtin (1).
Future work should select potentially interesting transcript pairs for further functional characterization to determine biological activity.
A Nod 19 family protein was another interesting transcript found to be up-regulated.
Huntington's disease (HD) is caused by an expanded CAG tract in the Interesting transcript 15 (IT15) gene encoding the 350 kDa huntingtin protein.
Another interesting transcript that exhibited increased abundance (2-fold; Table 2) in ABR17-transgenic plants was a putative mitogen-activated protein kinase (MAPK).
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