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The protein of interest was detected using ECL or ECL plus chemiluminescent Western blotting detection reagent and Hyperfilm ECL (Amersham plc., Little Chalfont, UK).
The RNA was blotted onto a positively charged nylon membrane (Roche) according to standard procedures, and the transcript of interest was detected using DIG-labeled antisense probes (DIG labeling and detection, Roche).
After incubation with a peroxidase-conjugated anti-mouse IgG secondary antibody, the protein of interest was detected using an ECL kit purchased from GE HealthCare (Piscataway, NJ).
No response pattern for topics covered by prior information and low current interest was detected.
After washing as described above, the protein of interest was detected with ECL-Plus (Amersham).
The protein band of interest was detected using enhanced chemiluminescence reagents (Amersham).
Similar(47)
The proteins of interest were detected using the ECL Plus detection system (Amersham, Piscataway, NJ).
The blots were washed again and the proteins of interest were detected using Amersham ECL Prime Western Blotting Detection Reagents (GE Healthcare) according to the manufacturer's instructions, and the chemiluminescence signal was then visualized with X-ray film.
In cases when no objects of interest are detected, again classical average metering is performed [17].
The region of interest is detected and then the signs of selected transform coefficients are scrambled.
If an abnormal event of interest is detected during the specified operations, then Eqs.
More suggestions(16)
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interest was inspected
interest was extracted
relevance was detected
interest was selected
attention was detected
interest was calculated
interest was collected
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com