Exact(2)
The over-expression of the genes of interest was confirmed using RT-PCR with gene-specific primers (Fig. 3A), and further, the levels of reaction products were quantified.
The identity of genes of interest was confirmed using NCBI tools, including BLAST.
Similar(58)
Changes in expression for three of four transcripts of interest were confirmed using real-time reverse transcription polymerase chain reaction.
The sequences of regions of interest were confirmed using PCR.
Methylation of interest loci was confirmed using pyrosequencing and/or combined bisulfite restriction analysis, and protein expression by immunohistochemistry.
For genes of interest, orthology to genes in Arabidopsis was confirmed using best reciprocal BLASTP ([ 52], E < 10-10).
Endothelial phenotype was confirmed using in vitro assays.
Validity of results (p-values) was confirmed using 1,000,000 bootstraps.
Selenomethionine incorporation was confirmed using MALDI-TOF mass spectrometry.
Data normality was confirmed using the Shapiro Wilk test.
The association was confirmed using data from the Health and Retirement Study.
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