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The relative expression of the gene of interest was analyzed using the 2-ΔΔCT method [ 48].
The association between OPCAB grafting and the long-term outcomes of interest was analyzed using adjusted survival curves and Cox proportional hazards modeling techniques.
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The spots of interest were analyzed using MALDI-TOF MS (Voyager-DE STR, Applied Biosystem, CA).
Expression of WEE1 and GAPDH genes as well as miRs of interest were analyzed using specific TaqMan® Assays in the 7900HT Real Time PCR system (Applied Biosystems, Foster City, CA) following standard protocol.
Several tryptic peptides of interest were analyzed using ESI MS/MS.
Five hundred base pairs upstream of the putative initiation codons of genes of interest were analyzed using default parameters.
Image reconstruction was performed on 25-μm voxels, a threshold was generated to define mineralized tissue and regions of interest were analyzed using the MicroView Analysis program (GE Healthcare Biosciences).
ERP mean amplitudes within each region of interest were analyzed using 4 (category: HA, HV, ENV, MEC) by 2 (hemisphere: left and right) repeated measures ANOVAs.
AEs of special interest were analyzed using standardized MedDRA queries (SMQs; narrow versions) and included hypersensitivity reactions, skin lesions, hepatic AEs, renal AEs, and pancreatitis.
Compounds of interest were analyzed using a Waters ACQUITY UPLC system (Waters Corp., Milford, MA, USA), consisting of a binary pump system, sample manager, column manager, and PDA detector (also from Waters Corp .. Waters MassLynx software v.4.1 was used for acquisition and data processing.
Transcriptional analysis was confirmed by protein expression levels for genes of interest using fluorescent immunohistochemistry. Data was analyzed using the GraphPad software package.
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