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The area of interest selected for correlations passes a few millimeters over the notch.
Real-time qPCR was used to validate RNA-seq data and genes of interest selected for further analysis.
Among the differentially expressed genes common to both studies were three genes of biological interest selected for validation in the previous study [ 39].
An example of typical regions of interest selected for the control and occlusion flaps are shown with dashed and solid lines respectively.
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With the "Point &ID" mode of the INCA Energy software, both points of interest and the areas of interest are selected for the EDX analysis.
In order to test whether selection pressure varied among different clades, one or two clades of interest were selected for testing.
Within an image cube (i.e. the whole 3D image obtained by stacking all the optical slices), ROIs (Regions of Interest) were selected for three types of positions: bulk solution, normal cell wall and dislocations.
Nine genes of functional interest were selected for validation by quantitative PCR (qPCR) analysis (Table 2).
Based on microarray- and pathway analyses, 6 genes of interest were selected for confirmation by q-PCR.
Using Image J v1.40 g software (National Institutes of Health, Bethesda, Maryland), background noise was removed and bands in the region of interest were selected for analysis.
Three genes of interest were selected for further study.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com